The placental regulatory factor involved in the asymmetric IgG antibody synthesis responds to IL-6 features

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Ulrich K. Laemmli · Nature · 1970 · 251.6K citations

Life Sciences, Microbiology, Molecular Biology +14

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Harry Towbin, T. Staehelin, J. Gordon · Proceedings of the National Academy of Sciences · 1979 · 53.8K citations · Full text

Life Sciences, Biochemistry, Molecular Biology +13

Continuous cultures of fused cells secreting antibody of predefined specificity

Georges Köhler, César Milstein · Nature · 1975 · 17.2K citations

Pharmacy, Cell Biology, Immunology +11

Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.

Eva Engvall, Peter Perlmann · PubMed · 1972 · 3.1K citations

Life Sciences, Biochemistry, Immunology +11

Purification and NH2-terminal amino acid sequence of a T-cell-derived lymphokine with growth factor activity for B-cell hybridomas.

Jacques Van Snick, Sylvie Cayphas, Anne Vink et al. · Proceedings of the National Academy of Sciences · 1986 · 685 citations · Full text

Life Sciences, Immunology, Molecular Biology +14