Analytical Biochemistry · 1995 · 37 citations · 0 references
Life SciencesMolecular BiologyMolecular GeneticsGene ExpressionGene Expression AnalysisTranscriptomicsCartilage BiologyCartilage MetabolismPolymerase Chain ReactionAggrecan BiologyArticular Cartilage ProteoglycansAssayCartilage QuantificationPcr-based DetectionProteoglycan BiologyQuantitative Polymerase Chain ReactionQuantitative Polymerase Chain Reaction Assays
A procedure has been developed to quantify the levels of aggrecan and link protein mRNAs in small amounts of various tissues, including cartilage, using the power of PCR to amplify extremely low levels of specific templates. The PCR protocol which was selected allows for a simple assay procedure, with standards in different tubes from the samples. This straightforward procedure is quantitative, inexpensive, and allows for many samples to be analyzed at one time.