Letters in Applied Microbiology · 2003 · 15 citations · 13 references
DentistryPeriodonticsPeriodontologyOral MicrobiologyPeriodontal MedicineSalivary DiagnosticsMicrobiological AnalysisOral Microbial DiagnosticsPeriodontal DiseasePeriodontal ExaminationPeriodontal MicrobiologySalivary MicrobiologyBleeding On Probing MonitoringPeriodontal Disease BiomarkersPeriodontal Disease DiagnosticsReal-time Polymerase Chain Reaction (Pcr)Real-time Polymerase Chain Reaction AssaysSaliva DiagnosticsSaliva-based DiagnosticsSalivary Nucleic Acid Detection
AIMS: The purpose of the present investigation was to assess the salivary levels of Streptococcus anginosus in periodontitis patients. METHODS AND RESULTS: The salivary levels of Strep. anginosus were assessed using real-time polymerase chain reaction (PCR). Streptococcus anginosus was detected in 28 of 37 (75.6%) of periodontitis patients and in three of the 20 (15%) healthy subjects. The mean values for bleeding on probing and probing depth in positive patients were statistically higher than those in negative patients. A significant decrease in Strep. anginosus levels was observed after periodontal treatment. CONCLUSIONS: Although the levels of Strep. anginosus are extremely low, they may reflect the status of periodontal health. SIGNIFICANCE AND IMPACT OF THE STUDY: Real-time PCR is a useful method for obtaining the relative quantities of Strep. anginosus from saliva samples and for monitoring the effect of therapy.
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Bacteriophage isolation from human saliva
Gilad Bachrach, M. Leizerovici-Zigmond, Amir Zlotkin et al. · Letters in Applied Microbiology · 2003 · 139 citations