Veterinary Record · 1994 · 150 citations · 0 references
Veterinary MedicineAnimal SciencesVeterinary MicrobiologyMolecular VirologyRuminant MedicineVirologyAntigen Detection AssaysLivestock Infectious DiseasesRuminant Infectious DiseasesSerological DiagnosisViral DiagnosticsAntibody Detection TestsAntigen Detection TestsImmunodiagnostic AssaysInfectious Disease DiagnosticsViral Antigen DetectionViral Disease DiagnosticsVirus Detection AssaysVirus-specific Antibody Detection
An immunocapture ELISA for the diagnosis of rinderpest and peste des petits ruminants is described. Monoclonal antibodies directed against non-overlapping antigenic domains on the nucleocapsid (N) were used to detect the virus N protein in supernatants from infected cells and in field specimens. The assay, which is very sensitive, can be performed in one hour on pre-coated plates. There was no cross reaction between the two viruses in the test and the N protein could be detected in infected cell supernatants kept at ambient temperature for one week. These results show that the ELISA is suitable for routine diagnosis of field samples.