PROTEOMICS · 2001 · 507 citations · 0 references
Life SciencesBiochemistryMolecular BiologyProtein BiochemistryProteomicsBottom-up ProteomicsMass Spectrometry-based ProteomicsProtein IdentificationProtein SeparationGel ElectrophoresisMass Spectrometry ProteomicsMass Spectrometry-based Protein IdentificationMatrix-assisted Laser Desorption Ionization Time-of-flight Mass SpectrometryMatrix-assisted Laser Desorption/ionizationPeptide Mass SpectrometryPeptide SeparationProtein Mass Spectrometry
In proteomics it is essential to be able to detect proteins separated by gel electrophoresis at high sensitivity. Silver staining is currently the most popular method. Here we present silver staining protocols that are optimized for staining sensitivity, peptide recovery and compatibility with digestion and mass spectrometry.