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A MICROMETHOD FOR MEASUREMENT OF RENIN IN THE PLASMA AND KIDNEY OF RATS
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1967
Year
HypertensionRenin ActivityRat SubstrateRenal FunctionBioanalysisNew MicromethodAnalytical ChemistryClinical ChemistryOsmoregulationAnimal PhysiologyVascular BiologyRenal PathophysiologyPharmacologyUrologyPhysiologyElectrophysiologyMedicineNephrologyKidney Research
A new micromethod for the measurement of renin activity and substrate in 0.1 ml of rat plasma and in 1 mg of rat renal tissue is described. In the procedure a rat substrate free of renin prepared from previously nephrectomized animals is used. Incubation is carried out in the presence of Dowex resin 50W-X2 (NH 4 + ) for 12 hours, at pH 6.5, with an excess of rat substrate. Angiotensin formed during incubation is measured by a pressor assay in the rat. The reproducibility of the procedure was excellent. Recovery of angiotensin in amounts from 10 to 500 mμg was 77%. This method permits repeated measurements of renin activity in rats during acute experiments and in any physiological procedure. It can readily be adapted to other species.