Science · 1985 · 284 citations · 19 references
GeneticsMolecular GeneticsPeripheral NerveCellular PhysiologyPeripheral Nervous SystemSocial SciencesNeuroregenerationGene SequenceGene StructureMolecular NeuroscienceMolecular CloningNgf-induced DifferentiationNervous SystemGene ExpressionCell BiologyDevelopmental BiologyNerve Growth FactorMolecular NeurobiologyTranscription FactorsMedicineNeural Stem Cell
Nerve growth factor (NGF) is essential for the development and differentiation of sympathetic or sensory neurons. A complementary DNA was cloned that corresponds to a gene sequence induced more than 50-fold in a cultured target cell line of pheochromocytoma cells (PC12 cells) 5 hours after the addition of NGF. The induced messenger RNA encodes a 90,000-dalton polypeptide that may represent one of the primary events in NGF-induced differentiation of neurons.
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