A Study of the Characteristics of Certain Rh Antibodies Preferentially Detectable by Enzyme Technique

Abstract

Abstract. Antibodies of various specificities which share the property of reacting by enzyme technique but either weakly or not at all by antiglobulin have been studied. They are found to occur among both IgG and IgM immunoglobulins. They are able to effect a positive antiglobulin reaction after being ‘built up’ by the formation of a globulin‐antiglobulin lattice on the red cells. In general the IgG antibodies require more ‘build‐up’ than the IgM. Various reasons for the failure of these antibodies to be detected by the antiglobulin technique as it is normally performed, are discussed.