International Journal of Dermatology · 2002 · 35 citations · 19 references
Life SciencesInfectious DiseasesMicrobiologyDiagnostic MicrobiologyMedical MicrobiologyMolecular DiagnosticsDiagnostic BacteriologyInfectious Disease Molecular TestingMycobacterial DiagnosticsPolymerase Chain ReactionTuberculosis DiagnosisTuberculosis Diagnostic TestingMicrobiological ConfirmationMycobacterium Tuberculosis InfectionPcr-based DetectionRapid Detection AssaysRapid Diagnostic AssaysRapid Molecular DiagnosticsTuberculosis DiagnosticsTuberculosis Molecular Testing
BACKGROUND: Most cutaneous tuberculosis lesions contain few bacilli, so identification of Mycobacterium tuberculosis in conventional laboratory tests is difficult. In vitro amplification of specific DNA sequences using polymerase chain reaction (PCR) has become a valuable tool in the rapid detection of slow-growing organisms like M. tuberculosis. AIM: To investigate the presence of M. tuberculosis DNA in cutaneous tuberculosis. METHODS: Twenty-two archival biopsy specimens diagnosed as cutaneous tuberculosis were investigated for the presence of M. tuberculosis DNA by PCR. Normal skin samples of 29 healthy patients were used as a control. RESULTS: Amplification of the M. tuberculosis DNA was observed in one of the cutaneous tuberculosis specimens and in a healthy control. CONCLUSION: Although PCR is a rapid diagnostic method, fixation procedures may decrease its sensitivity.
19