Further Studies on the Dissociation of Adult Mouse Tissue.

Abstract

1. A series of compounds known to complex Na+ or K+ has been studied with respect to their capacity to dissociate various tissues of the adult mouse. 2. Single cell suspensions of kidney, brain and connective tissue retaining the morphology of the various cellular types in situ may be obtained with the appropriate complexing agent. 3. The findings suggest that cells in most tissues are aggregated predominantly by coordination about K+. The extent to which cells may be aggregated by coordination through Na+ in some tissues cannot now be evaluated.