Lab on a Chip · 2011 · 32 citations · 4 references
EngineeringBiotechnologyMolecular BiologyBiomemsNanobiotechnologyNanomaterialsDna NanotechnologyDna PackagingMicrofluidic Device FabricationMicrofluidic DevicesMicrofluidic SystemsMicrofluidicsDna NanomachinesDna StretchingMetaphase Chromosome AnalysisMicrofluidic PackagingMicrofluidic PlatformsPressure-driven FlowPressure-driven Flow ControlTime-lapse Imaging
We describe the structure and operation of a micro/nanofluidic device in which individual metaphase chromosomes can be isolated and processed without being displaced during exchange of reagents. The change in chromosome morphology as a result of introducing protease into the device was observed by time-lapse imaging; pressure-driven flow was then used to shunt the chromosomal DNA package into a nanoslit. A long linear DNA strand (>1.3 Mbp) was seen to stretch out from the DNA package and along the length of the nanoslit. Delivery of DNA in its native metaphase chromosome package as well as the microfluidic environment prevented DNA from shearing and will be important for preparing ultra-long lengths of DNA for nanofluidic analysis.
4
Restriction mapping in nanofluidic devices
Robert Riehn, Manchun Lu, Yanmei Wang et al. · Proceedings of the National Academy of Sciences · 2005 · 211 citations