Composition of the Volatiles of<i>Citrus macroptera</i>var.<i>annamensis</i>and Evaluation of Bioactivity

Abstract

Abstract The distillation and subsequent extraction of the distillate obtained from the fresh peels of the fruits of Citrus macroptera var. annamensis afforded 120 mg of oil (yield 0.12 %). Analyses both by gas chromatography (GC) and gas chromatography- mass spectrometry (GC-MS) of the sample resulted in the identification of 25 terpenoids, predominantly monoterpene hydrocarbons, accounting for 97.0 % of the total oil, and limonene (73.5 %) as the major component. While the oil did not exhibit any in vitro free-radical-scavenging (DPPH), it showed considerable antibacterial activity against Bacillus cereus, B. subtilis, Escherichia coli and Staphylococcus aureus with the MIC values ranging from 1.25 to 5.0 mg/mL.