Chemically bonded maltosevia click chemistry as stationary phase for HILIC

Abstract

A HILIC stationary phase was synthesized through bonding maltose on silica particles via click chemistry. A set of representative polar compounds including sugars, amino acids and small peptides were employed to evaluate chromatographic properties of Click Maltose. The results illustrated the surface adsorption mechanism from the direct hydrogen bonding interaction between sugars and Click Maltose played an important role on retention behavior of sugars under HILIC mode. The chromatographic behavior of anions on Click Maltose was consistent with HILIC/WAX mechanism, while the retention of cations was depended on electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) mechanism. The Click Maltose stationary phase was successfully used in the separation of standard samples of polar compounds and complex samples of oligosaccharides from the natural product. The Click Maltose also exhibited good reproducibility and stability, which were helpful to further application. Our study confirmed the advantages and application potential of bonded oligosaccharides for HILIC separation.