Two Fluorescent Wavelengths, 440 _ex /520 _em nm and 370 _ex /440 _em nm, Reflect Advanced Glycation and Oxidation End Products in Human Skin Without Diabetes

Abstract

SAF measurements at 370(ex)/440(em) nm and 335(ex)/385(em) nm, except for pentosidine, which correlated with fluorescence at 370(ex)/440(em), correlate poorly with glycated and oxidatively modified protein in human skin and do not reflect actinic modification. A new fluorescence measurement (440(ex)/520(em) nm) appears to reflect AGEs and OPs in skin.