Journal of Pharmacy and Pharmacology · 1961 · 57 citations · 3 references
Life SciencesEndocrinologyPharmacologyAnalytical ToxicologyHormone BiochemistrySteroid BiochemistryBioanalytical Method ValidationFluorescence SpectroscopySteroid Hormone BiochemistrySteroid MetabolismSteroid PharmacologyFluorescence Emission SpectraFluorescence Excitation SpectraFluorescence QuantificationSex Steroid PhysiologySteroid DetectionSteroid Hormone Assays
Abstract The sensitive and specific fluorimetric methods introduced by Jensen (1952; 1953) for digitalis estimation were re-evaluated for the assay of cardiotonic steroids and other steroids of biological interest. Characteristics of the excitation and fluorescence spectra were determined. Using a modification of Jensen's original digitoxin method and a spectrofluorometer, a linear relation was demonstrated between fluorescence intensity and concentrations from 0.01 to 6 μg./ml. for both digitoxin and digoxin. Application of these methods to the assay of gitoxin and cholesterol would seem to be equally practicable. In addition, scillaren A and B and strophanthin K can be made to fluoresce weakly. On the other hand, ouabain, cholestanol, testosterone, and hydrocortisone showed no fluorescence. Possible mechanisms of the reactions are discussed
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