Concepedia

Cloning and expression in yeast Pichia pastoris of a biologically active form of Cyn d 1, the major allergen of Bermuda grass pollen

Penelope M. C. Smith, Cenk Suphioglu, Irwin J. Griffith, Kelly Theriault, R. B. Knox, Mohan B. Singh

Journal of Allergy and Clinical Immunology · 1996 · 71 citations · 40 references

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References

40

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.3K citations · Full text

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Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

Analytical Biochemistry · 1987 · 63.3K citations

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Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

Piotr Chomczyński, Nicoletta Sacchi · Analytical Biochemistry · 1987 · 46.9K citations

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Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.

Michael A. Frohman, Michael Dush, Gail R. Martin · Proceedings of the National Academy of Sciences · 1988 · 4.6K citations · Full text

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Signal sequences

Gunnar von Heijne · Journal of Molecular Biology · 1985 · 2.2K citations