Induction of Squamous Metaplasia, Dysplasia and Carcinoma <i>in situ</i> of the Mouse Tracheal Mucosa by Inhalation of Sodium Chloride Mist Following Subcutaneous Injection of 4‐Nitroquinoline 1‐Oxide

Abstract

Squamous metaplasia, dysplasia and carcinoma in situ (CIS) were induced in the ICR/Jcl mouse tracheal mucosa by exposure to a mist of 5% NaCl solution following single subcutaneous injection of 4-nitroquinoline 1-oxide (4-NQO). Either subcutaneous injection of 4-NQO or NaCl inhalation alone did not cause any marked change in the tracheal mucosa. The NaCl inhalation is considered to have promotion-like action on the mouse tracheal mucosa after administration of 4-NQO injection.