Influence of Preparative Procedure on the Purity of Chlorophyll Components as Shown by Absorption Spectra

Abstract

1. An improved method for preparation of chlorophyll component solutions with reproducible spectroscopic properties is presented. The method is rapid and simple; sucrose is used, both for filtration of precipitated pigment and for separation of components by adsorption. 2. The absorption spectrum in ether solution has been shown to be very sensitive to previous treatment of the solution. It is emphasized that drying must be avoided and that spectroscopic observations must be made very soon after purification. 3. The spectra of acidified chlorophyll solutions are presented, and their relation to ordinary degradation effects is discussed. Some comparisons with reported values are made.