Improved PCR method for detecting monoclonal immunoglobulin heavy chain rearrangement in B cell neoplasms.

Abstract

Deletions and extensive somatic mutations within the FrIII region may give false negative results with primers homologous to the region. A PCR using the method described, with a repertoire of primers homologous to the FrII and FrIII regions, will therefore increase the frequency of detection of monoclonality.