Biological Control of<i>Eutypa lata</i>on Grapevine by an Antagonistic Strain of<i>Bacillus subtilis</i>

Abstract

An isolate of Bacillus subtilis from grapevine wood inhibited in vitro growth of Eutypa lata, the causal organism of dieback in grapevines. On potato-dextrose agar, the bacterium caused 91.4% inhibition of mycelial growth of E. lata and 100% inhibition of ascospore germination. Ascospore germination correlated negatively with time of exposure to B. subtilis. Malformation of hyphae of E. lata occured in the presence of B. subtilis. At a concentration of ≥0.8 mg/ml, an antibiotic substance in an ethanol extract from B subtilis totally inhibited germination of ascospores E. lata (...)