Concepedia

Viable Cell Detection by the Combined Use of Fluorescent Glucose and Fluorescent Glycine

Hideaki Matsuoka, Kanenari Oishi, Masaaki Watanabe, Ikuko Kozone, Mikako Saito, Shizunobu Igimi

Bioscience Biotechnology and Biochemistry · 2003 · 15 citations · 9 references

DOIFull text

Open access

Concepts

Abstract

The combined use of a fluorescent glucose (2NBDG) and a fluorescent glycine (NBD-Gly) was tried for the detection of viable cells of significant foodborne pathogenic strains in addition to several Escherichia coli strains and coliforms. Thirty-five out of 41 strains showed marked uptake of 2NBDG but 6 strains were not able to take in 2NBDG. Five out of these 6 strains showed NBD-Gly uptake.

References

9

A novel fluorescent derivative of glucose applicable to the assessment of glucose uptake activity of Escherichia coli

Kazuaki Yoshioka, Hirokazu Takahashi, Tomoo Homma et al. · Biochimica et Biophysica Acta (BBA) - General Subjects · 1996 · 241 citations

, , +16

Intracellular Fate of 2-NBDG, a Fluorescent Probe for Glucose Uptake Activity, in<i>Escherichia coli</i>Cells

Kazuaki Yoshioka, Mikako Saito, Ki‐Bong Oh et al. · Bioscience Biotechnology and Biochemistry · 1996 · 149 citations

, , +17

Potential problems with fluorescein diacetate assays of cell viability when testing natural products for antimicrobial activity

Joanne M. Clarke, Michael R. Gillings, Nanda Altavilla et al. · Journal of Microbiological Methods · 2001 · 75 citations

, , +7

The ability of direct fluorescence-based, two-colour assays to detect different physiological states of oral streptococci

E. Decker · Letters in Applied Microbiology · 2001 · 70 citations

, , +15

The use of NBD chloride (7 chloro-4-nitrobenzo-2-oxa-1,3-diazole) in detecting amino acids and as an N-terminal reagent

Roger S. Fager, Charles B. Kutina, E. W. Abrahamson · Analytical Biochemistry · 1973 · 69 citations

, , +4