Methods and Equipment for the Study of the Incorporation of Phosphorus by Intact Barley Plants in Experiments of Short Duration

Abstract

Paper partition chromatography has been used for the separation of organic phosphorus compounds extracted from roots after short periods of absorption of radioactive phosphorus by whole barley plants. Methods of extraction, fractionation, and identification of the compounds are described. Equipment has been designed to record rapidly the activity along the length of the chromatograms. Salient features are congruence between chromatograms and record, high sensitivity and provision for automatic change of sensitivity.