An Improved Chromatographic Procedure for Quantitation of Human Fetal Hemoglobin

Abstract

The DW-cellulose chromatographic procedure which uses glycine-KCN-NaC1 solutions as Developers and a NaCl gradient to elute the hemoglobin zones was found to be useful for the quantitation of Hb F In samples containing Hb A, provided the level of Hb F exceeds 2%. The method has been evaluated through a study of artificial mixtures and of blood samples from patients with various disorders. The data have been compared with results obtained by four other procedures that are used for the quantitation of Hb F.