Nitrosative stress by cyclosporin A in the endothelium: studies with the NO‐sensitive probe diaminofluorescein‐2/diacetate using flow cytometry

Abstract

In this work, we provide data showing that intracellular NO can be detected by flow cytometry with DAF-2/DA and suggesting two potential mechanisms (transcriptional and post-translational) for the intracellular accumulation of NO in vascular endothelial cells exposed to the immunosuppressant CSA: