AN ADDITIONAL PURIFICATION STEP FOR A METHOD FOR ESTIMATING OESTRIOL, OESTRONE AND OESTRADIOL-17β IN HUMAN URINE

Abstract

SUMMARY 1. A modification of a method for estimating urinary oestrogens is described and its accuracy confirmed. 2. For most urine specimens the modified method gives practically the same results as the original method. In large series of comparisons, however, small but significant differences are found in the amounts of oestrone and oestriol measured by the two methods. 3. Interference caused by administration of certain drugs or by the secretion of large amounts of neutral steroids is reduced or abolished by using the modified method. The amounts of impurities in the final urine extracts are reduced by approximately 50%.