Publication | Closed Access
Stimulation by Insulin<i>in Vitro</i>of Incorporation of (<sup>35</sup>S)Sulfate and (<sup>14</sup>C)Leucine Into Protein-Polysaccharide Complexes, (<sup>3</sup>H)Uridine Into RNA, and (<sup>3</sup>H)Thymidine Into DNA of Costal Cartilage from Hypophysectomized Rats
41
Citations
0
References
1968
Year
GlycobiologyActinomycin Inhibited IncorporationMolecular BiologyInsulin SignalingGastrointestinal Peptide HormoneLeucine IncorporationInsulin DeliveryProtein-polysaccharide ComplexesUridine Into RnaMolecular PhysiologyGrowth HormoneBiochemistryEndocrinologyCostal CartilagePharmacologyNatural SciencesDiabetesPhysiologyCellular BiochemistryMetabolismMedicineThymidine Into Dna
The effects of insulin in vitro on incorporation of (35S)sulfate and (14C)leucine into protein-polysaccharide complexes (PP), (3H)uridine into ribonucleic acid (RNA), and (3H) thymidine into desoxyribonucleic acid (DNA) of costal cartilage from hypophysectomized rats were determined. Tissue was incubated in a medium containing 14 amino acids and glucose. Insulin stimulated incorporation of both sulfate and leucine into PP. The effect upon leucine incorporation was detected first. The action of the hormone was most striking in a component of PP relatively resistant to extraction in water. Either puromycin or actinomycin inhibited incorporation of sulfate and leucine into PP in the presence or absence of insulin. At 10−6m concentration of both antibiotics the effect of puromycin was more profound. Insulin also increased incorporation of both uridine into RNA and, later, thymidine into DNA. (Endocrinology82: 493, 1968)