<i>Helicobacter pylori</i> Adhesin Binding Fucosylated Histo-Blood Group Antigens Revealed by Retagging

TLDR

Helicobacter pylori causes peptic ulcer disease and adheres to gastric epithelium via the fucosylated Lewis b histo‑blood group antigen. The study proposes a BabA‑based vaccine strategy to target virulent type I H. pylori strains. BabA, the Le b‑binding adhesin, was purified using receptor activity–directed affinity tagging.

Abstract

The bacterium Helicobacter pylori is the causative agent for peptic ulcer disease. Bacterial adherence to the human gastric epithelial lining is mediated by the fucosylated Lewis b (Le b ) histo-blood group antigen. The Le b -binding adhesin, BabA, was purified by receptor activity–directed affinity tagging. The bacterial Le b -binding phenotype was associated with the presence of the cag pathogenicity island among clinical isolates of H. pylori . A vaccine strategy based on the BabA adhesin might serve as a means to target the virulent type I strains of H. pylori.