Correction of instrument spreading in gel‐permeation chromatography

Abstract

Abstract Present methods of correcting instrument spreading (resolution correction) in GPC are either too cumbersome to use or inaccurate when the correction is large. Two new methods which are both accurate and simple to use are presented in this work. The first method using the technique of Fourier analysis is more general and can be used to correct non‐Gaussian instrument spreading. The second method using a fourth‐degree polynomial requires a Gaussian instrument spreading function. The instrument spreading function may vary with respect to the elution volume in both methods.