Reproducibility of Endothelial Assessment during Corneal Organ Culture: Comparison of a Computer-Assisted Analyzer with Manual Methods

Abstract

Manual counting shows systematic underestimation of ECD with high interobserver variability. The analyzer in automated mode overestimates ECD and is absolutely unreliable. Detection of cell contours by the specific algorithm, combined with manual correction by a skilled technician, appears to be the most reliable method of ECD and morphometry determination.