Ratio of Digestive Enzymes in the Chick Pancreas

Abstract

The ratio of exocrine enzymes was determined in extracts from pancreata of broiler chickens. The proteins were separated by ion-exchange chromatography to determine the elution pattern and the relative concentration of the various enzymes. Amylase was about 28.9%, the three chymotrypsinogens about 20%, and a single, anionic form of trypsinogen about 10% of the total protein. Procarboxypeptidases A and B, proelastase, lipase, and a secretory trypsin inhibitor were also present.