A Gold Nanoparticle‐Based Aptamer Target Binding Readout for ATP Assay

Abstract

A gold nanoparticle-based detection strategy for ATP assays. In the absence of ATP, gold nanoparticles are not stabilized by the rigid duplex, thus they are readily aggregated by salt (solution displaying blue colors); in contrast ATP induces a duplex-to-aptamer structural switching, liberating a random coil-like ssDNA. Gold nanoparticles are stabilized by the liberated ssDNA, showing high resistance to salt-induced aggregation (solution staying in red). Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2089/2007/c2256_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.