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Inverting Enantioselectivity of Burkholderia cepacia KWI-56 Lipase by Combinatorial Mutation and High-throughput Screening Using Single-molecule PCR and In Vitro Expression

Vitro ExpressionBiosynthesisEngineeringBiochemistryCombinatorial MutationKwi-56 LipaseLipid ResourceBiotechnologyNatural Product BiosynthesisMicrobiologyEnzymatic Modification