DOUBLE ISOTOPE DERIVATIVE ASSAY OF ALDOSTERONE, CORTICOSTERONE, AND CORTISOL

Abstract

A method of double isotope derivative assay of aldosterone, corticosterone, and cortisol is described. It involves acetylation with acetic- 3 H anhydride of high specific activity followed by the purification of the steroids through four paper chromatographies and the formation of a derivative by chromium trioxide oxidation. The method is particularly suited to the quantitative measurement of these steroids in the incubation media of either rat or beef adrenal slices.