Experimental Biology and Medicine · 1972 · 33 citations · 0 references
Life SciencesCell BiologyImmunologyCell AdhesionHemostasisInflammationAdhesion MoleculesCell-cell AdhesionLeukocyte AdhesionLeukocyte RecruitmentCellular Adhesion MoleculesDivalent Ion EffectsLeukocyte Adhesion MoleculesLeukocyte AggregationLeukocyte InteractionsLeukocyte Surface MarkersLeukocyte Toxicity
A new method for quantitation of leukocyte adhesion to glass is described. Using this method, it was possible to demonstrate the adverse effects of low temperature, sodium arsenite, divalent cation chelation, marked hyperosmolality, and heat inactivated plasma on leukocyte adhesiveness. Adhesion was enhanced by increased Mg2+ concentration, minimally increased concentrations of sodium chloride, brief exposure to sodium arsenite, and suspension of leukocytes in a plasma-free environment.