Measurement of transverse and axial apparent dispersion coefficients in packed beds

Abstract

Abstract The axial and transverse apparent dispersion coefficients of three solvents in two packed chromatographic columns were determined by pulsed‐field‐gradient nuclear‐magnetic resonance in a range of mobile phase velocity. The column beds were packed with 5‐ μm particles of porous C18 silica or 30‐μm particles of silica. The solvents used were methanol and acetonitrile (in an 80:20 ACN/water solution) in the former case and acetone in the latter. The coefficients were determined over a range of particle Peclet numbers from less than 0.1 to approximately 10 in the former case and 40 in the latter. The data obtained with short dispersion times were fitted to the correlations suggested by Giddings, Horvath and Lin, Huber, and Knox. These data agreed well only with the Giddings equation.