Cloning vectors that yield high levels of single-stranded DNA for rapid DNA sequencing

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.1K citations · Full text

Dna, Engineering, Dna Analysis +20

Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977.

Francisco Bolívar, Rodriguez Rl, Greene Pj et al. · PubMed · 1992 · 4.7K citations

Engineering, Genetic Engineering, Vehicle Technology +2

Construction and characterization of new cloning vehicle. II. A multipurpose cloning system

Francisco Bolívar, Raymond L. Rodriguez, Patricia J. Greene et al. · Gene · 1977 · 3.9K citations

Cloning, Engineering, Genetic Engineering +1

A rapid boiling method for the preparation of bacterial plasmids

David S. Holmes, Michael E. Quigley · Analytical Biochemistry · 1981 · 3.3K citations

Rapid Boiling Method, Bacteriophage, Bacteriology +2

A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments

Joachim Messing, Jeffrey Vieira · Gene · 1982 · 2.9K citations

New Pair, Dna Sequencing, Natural Sciences +13