Stepwise Self‐Assembly of a Protein Nanoarray from a Nanoimprinted Poly(Ethylene Glycol) Hydrogel

Abstract

Ultraviolet nanoimprint lithography is used to fabricate nanopatterns of poly(ethylene glycol) (PEG) hydrogel on gold substrates for the construction of protein nanoarrays. A protein nanoarray with a minimum feature size of 100 nm is constructed in the nanowell of the PEG hydrogel by self-assembly with a biotinylated PEG-disulfide, streptavidin, and biotinylated antibody, sequentially (see figure), and confirmed by varying the nanowell height between each step in both solution and air. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2296/2008/z700865_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.