ESTRADIOL RADIOIMMUNOASSAY WITHOUT CHROMATOGRAPHY: PROCEDURE, VALIDATION AND NORMAL VALUES

Stanley G. Korenman, Reggie H. Stevens, Leslie Ann Carpenter, Marianne Robb, G. D. Niswender, Barry M. Sherman

The Journal of Clinical Endocrinology & Metabolism · 1974 · 385 citations · 0 references

Concepts

Abstract

Employing a highly specific estradiol antiserum, an assay for the hormone was developed and validated which does not require a chromatographic step. The mean male E2 value was 20.5 pg/ml and for post-menopausal women, 10.5 pg/ml. Values for the normal human menstrual cycle centered at the estradiol peak are given.