Phosphate Mediated Regulation of Cinnamoyl Putrescine Biosynthesis in Cell Suspension Cultures of<i>Nicotiana tabacum</i>

Abstract

The accumulation of cinnamoyl putrescines by cell suspension cultures of NICOTIANA TABACUM was enhanced manifold by phosphate limitation of the culture medium while growth was reduced under such conditions. The enhanced product formation was preceded by a large increase and subsequent decline of phenylalanine ammonia lyase activity and a smaller increase of the activity of ornithine decarboxylase. Phosphate concentrations commonly used in cell culture media suppressed the product accumulation.