Lab on a Chip · 2010 · 199 citations · 24 references
EngineeringBioengineeringBiomedical EngineeringAnalytical ChemistryBiomemsClinical BiochemistryBiomedical InstrumentationMedical InstrumentationMicrofluidic BiosensorsMicrofluidicsOptical BiosensingOptical BiosensorsBioanalytical MicrosystemsBlood Analysis MicrodevicesImmunoassay InstrumentationMicrofluidic Diagnostic DevicesMicrofluidic Diagnostic PlatformsMicrovalvesOptical DetectionWhole Blood Separation
We report a fully integrated device that can perform multiple biochemical analyses and sandwich‑type immunoassays simultaneously on a single disc. The device uses a disposable lab‑on‑a‑disc with freeze‑dried reagents, laser‑irradiated ferrowax microvalves, and centrifugal microfluidics to automate plasma separation, metering, mixing, incubation, washing, and detection, with optical detection at 10 wavelengths. Within 22 min the disc reports analyte concentrations using only 350 µL of blood versus 3 mL, delivering results faster than conventional laboratory analysis while eliminating the need for trained operators or expensive instruments.
We report a fully integrated device that can perform both multiple biochemical analysis and sandwich type immunoassay simultaneously on a disc. The whole blood is applied directly to the disposable “lab-on-a-disc” containing different kinds of freeze-dried reagents for the blood chemistry analysis as well as reagents required for the immunoassay. The concentrations of different kinds of analytes are reported within 22 min by simply inserting a disc to a portable device. Using the innovative laser irradiated ferrowax microvalves together with the centrifugal microfluidics, the total process of plasma separation, metering, mixing, incubation, washing, and detection is fully automated. The analyzer is equipped with an optical detection module to measure absorbances at 10 different wavelengths to accommodate the various kinds of reaction protocols. Compared to the conventional blood analysis done in clinical laboratories, it is advantageous for point-of-care applications because it requires a smaller amount of blood (350 μL vs. 3 mL), takes less time (22 min vs. several days), does not require specially trained operators or expensive instruments to run biochemical analysis and immunoassay separately.
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Design of a Compact Disk-like Microfluidic Platform for Enzyme-Linked Immunosorbent Assay
Siyi Lai, Shengnian Wang, Jun Luo et al. · Analytical Chemistry · 2004 · 423 citations
A fully automated immunoassay from whole blood on a disc
Beom Seok Lee, Jung‐Nam Lee, Jung‐Nam Lee et al. · Lab on a Chip · 2009 · 301 citations
Engineering, Biomedical Engineering, Infectious Diseases +17