Preparation of Native Tobacco Mosaic Virus Protein by Continuous Free-flow Electrophoresis

Abstract

Conditions have been standardised for the preparation of nucleic acid-free protein from several strains of tobacco mosaic virus by the method of continuous free-flow electrophoresis. The procedure is simple and it yields large quantities of protein in a relatively short time. Due to the native character of the isolated proteins, they can be used advantageously in physicochemical and biological studies.