Cloning and Characterization of Full-Length cDNA Encoding Cell-Wall Invertase from Maize

Abstract

Invertases (P-fructofuranosidase, EC 3.2.1.26) have been studied in many organisms, including plants. At least two forms of the enzyme, soluble and particulate, are a common feature to a11 organisms. The soluble form is predominantly localized to vacuoles and cytoplasm and the particulate form, in a11 cases tested, is ionically bound to the cell wall (hence, cell-wall-bound form) and is readily extractable with high salt concentration Each of the two forms of invertase is known to have severa1 isozymes