Novel quantitative TaqMan real-time PCR assays for detection of <i>Cryptosporidium</i> at the genus level and genotyping of major human and cattle-infecting species

Abstract

No molecular-based method was yet available for the quantitative detection of C. andersoni and the cluster formed by C. bovis, Cryptosporidium ryanae and Cryptosporidium xiaoi. This work provides a novel tool to evaluate the parasite load from domestic ruminants and humans, and to improve assessment and management of microbial risk through better appraisal of the origin and fate of faecal pollutions.