Isolation and characterization of polymorphic microsatellites in diploid strawberry (<i>Fragaria vesca</i> L.) for mapping, diversity studies and clone identification

Abstract

Abstract This study reports the development and characterization of 10 polymorphic microsatellite primer pairs in wild strawberry ( Fragaria vesca ). The primers were designed from a genomic library enriched for di‐, tri‐ and tetranucleotide repeats from F. vesca ‘Reugen’. They showed single locus polymorphism in a set of nine F. vesca accessions; two to six alleles were detected per locus. The level of polymorphism in F. vesca was surprisingly low, although three pairs of primers were sufficient to distinguish between most accessions.