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Domains of the parathyroid hormone (PTH) receptor required for regulation by G protein-coupled receptor kinases (GRKs)11Abbreviations: PTH, parathyroid hormone; PTHrP, PTH-related protein; cAMP, cyclic AMP; Tris, tris(hydroxymethyl)-aminomethane; Kd, dissociation constant; Bmax, maximal number of specific binding sites; PLC, phospholipase C; G-protein, guanine nucleotide regulatory protein; DMEM, Dulbecco’s modified Eagle’s medium; RT-PCR, reverse transcription-polymerase chain reaction; IBMX, isobutylmethyl xanthine; C-terminus, carboxyl-terminus; GPCR, G protein-coupled receptor; GRK, G protein-coupled receptor kinase; and D-PBS, Dulbecco’s phosphate-buffered saline.