Production of testosterone from progesterone by rat testicular microsomes without release of the intermediates 17alpha-hydroxyprogesterone and androstenedione

Abstract

It has been shown that during the in vitro conversion of progesterone to androstenedione, 17α-hydroxy-progesterone is not an obligatory intermediate which equilibrates with freely diffusible steroids in the incubation medium. Recently a cytochrome P-450 was purified that catalyzed, in addition to hydroxylase/lyase activities, reduction of androstenedione to testosterone. In order to determine whether progesterone could be transformed to testosterone without both intermediates (17α-hydroxyprogesterone and androstenedione) being equilibrated with steroids in the medium, several double-label double-substrate experiments were performed. When rat microsomes were incubated with an equimolar mixture of [14C]progesterone and 17α-hydroxy[3H]progesterone, androstenedione was isolated with a 11-fold higher 14C/3H ratio than 17α-hydroxyprogesterone, indicating that androstenedione could not be produced from free, diffusible 17α-hydroxyprogesterone. Incubation of an equimolar mixture of 17α-hydroxy[3H]progesterone and [14C]androstenedione with testicular microsomes resulted in the incorporation of 3–4-fold more 17α-hydroxyprogesterone into testosterone than of androstenedione, although the latter is the immediate precursor of testosterone. In an experiment in which equimolar concentrations of [3H]progesterone and [14C]androstenedione were incubated with testicular microsomes, the large pool of progesterone inhibited competitively lyase activity, but still the label of progesterone was incorporated into testosterone to the same extent as that of androstenedione. These results indicate that testosterone can be produced by immature rat testicular microsomes from added progesterone on an organized unit without the intermediates equilibrating with the incubation medium.