Cloned Poliovirus Complementary DNA Is Infectious in Mammalian Cells
Science · 1981 · 690 citations · 12 references
Poliovirus Complementary DnaViral ReplicationReverse GeneticsNatural SciencesPathogenesisGenetic EngineeringDna ReplicationVirologyMolecular BiologyPoliovirus GenomeRna GenomeMicrobiologyMedicineVirus GeneViral GeneticsInfectious Poliovirus
A complete, cloned complementary DNA copy of the RNA genome of poliovirus was constructed in the Pst I site of the bacterial plasmid pBR322. Cultured mammalian cells transfected with this hybrid plasmid produced infectious poliovirus. Cells transfected with a plasmid which lacked the first 115 bases of the poliovirus genome did not produce virus.
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