Determination of Cucumber Mosaic Virus Titer in Muskmelon by Enzyme-linked Immunosorbent Assay and Correlation with Aphid Transmission

Abstract

Percentage transmission by individual Aphis gossypii and spectrophotometric readings in indirect enzyme-linked immunosorbent assay (ELISA) were used to estimate cucumber mosaic virus (CMV) titers in muskmelon plants over a 7-wk period. Results indicated a high correlation between the two techniques with two distinct CMV isolates. One week after inoculation, the frequency of aphid transmission of CMV-243 (virulent isolate) was significantly higher than that of CMV-241 (mild isolate), and ELISA absorbance readings at 405 nm were significantly higher for isolate CMV-243 (...)