Use of Anhydrous Hydrogen Fluoride in Peptide Synthesis. II. Procedures for the Syntheses of Simple Peptides

Abstract

Abstract Detailed conditions for handling simple peptide-derivatives with anhydrous hydrogen fluoride (HF) were presented. Since HF has a strong affinity for peptides, the removal of excess HF from the reaction products is rather difficult, but is essential to obtain the product as good crystals. Syntheses of the following peptides were carried out successfully as examples of the HF procedure: tosyl-l-phenylalanyl-l-phenylalanine, l-leucylglycine, l-arginylglycine, carbobenzoxy-l-prolyl-l-leucylglycine ethyl ester, l-aspartyl-l-phenylalanine amide, and BOC-β-alanyl-l-tryptophyl-l-methionyl-l-aspartyl-l-phenylalanine amide.