Publication | Closed Access
THE SEPARATION AND PURIFICATION OF HUMAN LUTEINIZING AND THYROTROPHIC HORMONES
37
Citations
0
References
1964
Year
EngineeringHuman GrowthHormone ActivityReproductive EndocrinologySummary Ion-exchange ChromatographyPituitary GlandBioanalysisAnalytical ChemistryPituitary DiseaseChromatographyBiochemistryEndocrine MechanismHormonal ReceptorEndocrinologyBiomolecular EngineeringHuman Pituitary FractionPhysiologyReceptor BiologyThyroid HormoneMedicineEndocrine Research
SUMMARY Ion-exchange chromatography was used to further purify a human pituitary fraction rich in thyrotrophic and luteinizing hormone activities. Approximately twofold concentration of both activities was obtained by chromatography on IRC-50 at pH 7·5, but the hormones were not separated. Subsequent chromatography on DEAE-cellulose at pH 9·5 led to a tenfold concentration of the luteinizing hormone in a fraction practically free of thyrotrophic activity and to a fourfold concentration of the thyrotrophic hormone in a fraction still exhibiting substantial luteinizing hormone activity.